Juniper Publishers-Open Access Journal of Toxicology

JUNIPER PUBLISHERS-OPEN ACCESS JOURNAL OF TOXICOLOGY Development of Respiratory Mucosal Irritation and Toxicity Screening Methods: Comparison of MTT and Colorimetric Resazurin-Based Assay Authored by Remigius Uchenna Agu Abstract Availability of reliable and reproducible assay methods that use simple tools will facilitate research especially in resource-poor settings. The purpose of this study is to evaluate colorimetric resazurin reduction assay (CRA) as a possible tool in respiratory mucosal cell irritation and toxicity screening. It compared CRA with another well-accepted colorimetric cell viability assay method (3- [4, 5-Dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolinium bromide {MTT} assay). Cell viability results of treated cells were obtained using both assay methods and used to determine Z' factor, signal-to-background (S/B) ratio and IC 50 values. The results show all the Z ' values obtained were positive except values from CRA for cells trea...

JUNIPER PUBLISHERS-OPEN ACCESS JOURNAL OF TOXICOLOGY Toxicity of Cymbopogon proximus (Maharaib) Oil Extract to Newzealand Rabbits Authored by Medani AB Abstract The clinical, pathological, hematological and biological changes in Newzealand rabbits’ groups given daily oral doses of 0.1, 0.25 and 0. 5 ml/kg body weight/day of Cpmbopogon proximus oil extract were investigated in experiment duration for 21 days. Other than the dose co-related mortality rates, the clinical signs were observed daily after dosing to be low appetite and nervous signs including restlessness and increased consciousness. Pulmonary excretion of the oil extract led to bloody spots on the lungs, lymphocyte infiltration, congestion and edema. Renal glomeruli manifested lymphocyte infiltration in addition to shrinkages and easinophilic material in the medulla, if considered with the corticomedullary generalized necrosis and the significant changes in urea, they may explain the renal dysfuncti...